Invitrogen clonase ii manual

 · the intermediate entry clone, refer to the Gateway® Technology with Clonase® II manual for a one-tube protocol. Although this protocol allows you to generate expression clones more rapidly than the standard BP reaction followed by the LR reaction, fewer expression clones will be obtained (generally 10–20% of the total number of entry clones).File Size: KB. gateway technology clonase ii. user guide For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of blunt-end PCR products into an entry vector.  · the intermediate entry clone, refer to the Gateway® Technology with Clonase® II manual for a one-tube protocol. Although this protocol allows you to generate expression clones more rapidly than the standard BP reaction followed by the LR reaction, fewer expression clones will be obtained (generally 10–20% of the total number of entry clones).File Size: KB. gateway technology clonase ii.

Gateway Manual Invitrogen 6,,, and 6,, and/or other pending U.S. and foreign patent applications owned by Invitrogen Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased Gateway ® BP Clonase™ II Gateway BP Clonase II Enzyme Mix - Thermo Fisher Scientific. LR Clonase™ II enzyme mix catalyzes in vitro recombination between an entry clone (attL-flanked “gene”) and an attR-containing destination vector to generate an attB-containing expression clone. Store Gateway™ LR Clonase™ II enzyme mix at –20°C (non-frost-free freezer) for up to 6 months. For long-term storage, store at –80°C. LR Clonase II Plus Enzyme Mix. Single mix of enzyme and reaction buffer. Highest cloning efficiency for single and multiple fragment cloning. Optimized for difficult cloning reactions. Works with MultiSite Gateway Pro Technology. Easy to use single mix format ensures enzyme stability. Convenient 10 µl reaction set up. Very good.

B. Transfer gene from Entry Clone into Destination Vector with LR CLONASE Enzyme Protocol for Constructing a GATEWAy Destination Vector. I've been following the protocol step by step, but I don't get colonies. I use the LR clonase II plus from Thermofisher. The plasmids that I use are from the. Enzymes. BP Clonase enzyme mix (including BP Reaction buffer); (Invitrogen - - 20 rxns); Proteinase K solution (2 mg/ml); LR Clonase enzyme mix.