Invitrogen lr clonase ii manual

Technology, MultiSite Gateway® and MultiSite Gateway® Pro manuals, available at www.doorway.ru Quality Control LR Clonase™ II Plus enzyme mix is functionally tested in a hour reaction to combine 4 fragments into one DEST vector, followed by a transformation assay. References 1. Landy, A. () Ann. Rev. Biochem. 58, File Size: KB.  · Invitrogen Manual Version D (link updated August). Invitrogen™ Gateway™ LR Clonase™ II enzyme mix is a proprietary enzyme and buffer formulation containing the bacteriophage lambda recombination proteins Integrase (Int) and Excisionase (Xis), the E. coli-encoded protein Integration Host Factor (IHF). Catalog number: Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and a destination vector (containing att R sites) to generate an expression clone. Gateway® LR Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.

The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months. BP Clonase™ II enzyme mix catalyzes in vitro recombination between an attB-PCR product (or attB-containing expression clone) and an P-containing donor vector to generate an attL-containing entry clone. Store Gateway® BP Clonase™ II enzyme mix at ºC (non-frost-free freezer) for up to 6 months. For long-term storage, store at ºC. Catalog number: Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and a destination vector (containing att R sites) to generate an expression clone. Gateway® LR Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.

The following protocol is fast and will remove DNA < bp. 1. Add μl of TE to a μl amplification reaction. 2. Add μl of 30% PEG /. The Gateway® system utilizes in vitro site-specific recombination, which offers For the reaction, you'll need Invitrogen's “LR Clonase II Plus“ enzyme. User Protocol TB Rev. D JN. Page 6 of Gateway® LR Reaction. Gateway® LR Clonase™ II Enzyme Mix is included with Gateway® Nova DEST™ Expression.