Invitrogen neon manual

The Neon® Transfection System is: • Efficient —up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells. • Flexible —easily transfect from 2 × 10 4 cells to 6 × 10 6 cells per reaction. • Simple —easy to use, with a single reagent kit for all cell types. • . Fill the Neon ™ Tube with 3 mL of Electrolytic Buffer (use Buffer E for the 10 μL Neon ™ Tip and Buffer E2 for the μL Neon ™ Tip). Note: Make sure that the electrode on the side of the tube is completely immersed in buffer. 3. Insert the Neon ™ Tube into the Neon Pipette Station until you hear a click sound (Figure 1). Figure 1 File Size: 75KB. life technologies and/or its affiliate(s) disclaim all warranticorporation es with respect to this document, expressed or implied, including but not limited to those of merchantability, fitness for a particular purpose, or non-infringement. to the extent instruction manual: 1. neon File Size: 1MB.

Neon Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 x 10 4 cells. The Neon system uses a simple 3-step transfection procedure. The transfection occurs in the Neon Pipette tip. Load mix of harvested cells and molecules to be delivered (e.g., DNA, RNA, protein) into the Neon Pipette tip. Plug the pipette into position in the Neon transfection device; select your protocol, and press Start. Neon™ Transfection System 10 μL Kit. The Neon™ Transfection System 10 µL Kit is designed specifically for use with the Neon™ Transfection System. Use this kit for transfection volumes of 10 µL, containing 5 × 10 4 –2 × 10 5 adherent cells or 1 × 10 5 –5 × 10 5 suspension cells. Cells that have been transfected using the.

Invitrogen Neon™ Transfection SystemNeon™ transfection technology enables highly efficient delivery of nucleic acids into virtually all. Short title: A cost-effective approach for Neon microporation the NeonTM Transfection System (Invitrogen), a device that is increasingly gaining in. 18 лип. р. Availability of an efficient transfection protocol is the first determinant The Invitrogen ™ Neon Transfection System delivers DNA, RNA.