1 g 1 g 1 g. mg: 2°C to 8°C 2°C to 8°C 2°C to 8°C. 2°C to 8°C: Product Use For Research Use Only. Assays used the Superscript III Platinum One-Step Quantitative RT-PCR System (Invitrogen, Carlsbad, CA) in μl reaction mixture volumes comprising μl Superscript III RT Taq mix, 5 μl ×2 Reaction Mix (containing mM each deoxynucleoside triphosphate [dNTP]), mM MgSO4, μM each primer, μM probe, and nuclease-free water. Description. SuperScript III Reverse Transcriptase (RT) is active at 50°C and has a half-life of minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples.
Description. SuperScript III Reverse Transcriptase (RT) is active at 50°C and has a half-life of minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Superscript III Platinum Taq (Invitrogen #) Betaine, 5M (Sigma #BVL) if SS III enzyme kit is used. Autoclaved PCR tubes ( ml, thin-walled) Rubella Genotyping RT-PCR Kit, (primers and control RNA). Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich.
A), SuperScript® II and SuperScript® III (Invitrogen Cat. primers (R) according to the manufacturer's instructions for each reverse transcriptase. ProtoScript II – NEB ProtoScript II First Strand cDNA Synthesis Kit and random primer mix; SuperScript II – Invitrogen SuperScript First Strand Synthesis System. SUPERSCRIPT II RNase H. _. Reverse Transcriptase (U.S. Patent 5,,) is purified to near homogeneity from E. coli containing the pol gene of Moloney.
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