Invitrogen taq polymerase manual

Use a high-fidelity Taq polymerase (e.g., Platinum Taq, Life Technologies) to amplify the target sequence and, using gel electrophoresis, verify the integrity and concentration of the PCR product. 3. Mix the PCR product in the appropriate molar ratios with the 5′- or 3′-TOPO-adapted elements to create a linear construct.  · Titanium® Taq PCR Kits User Manual PT www.doorway.ru PR0X Clontech Laboratories, Inc. A Takara Bio Company Page 6 of 11 5. T/A Cloning: Titanium Taq PCR products are compatible with T/A cloning methods. For best results, please observe the T/A cloning tips in Section V.B. Platinum™ Taq DNA Polymerase is a recombinant Taq polymerase complexed with a proprietary antibody that blocks the polymerase activity at ambient temperatures. ∤ Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic “hot start” and offering increased sensitivity, specificity, and yield.

Platinum® Taq DNA High Fidelity Polymerase (5 U/µL) µL 1 U* * 1 U is sufficient for amplifying most targets. In some cases, more enzyme may be required (up to units). Use 1 U of enzyme for targets 12 kb. Mix and briefly centrifuge the components. 3 Add template DNA and primers. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter. Highlights. • Thermostable —half life is more than 40 min at 95°C. • Generates PCR products with 3'-dA overhangs. • Supplied with two buffers —10X Taq Buffer with KCl and 10X Taq Buffer with (NH 4) 2 SO 4. Invitrogen platinum taq manual. Без рубрики. Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed.

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. Upgrade to Invitrogen™ Platinum™ SuperFi™ II DNA Polymerase: >X higher fidelity than Taq, versatile and now enables universal primer annealing. The extra time, effort, and contamination risks associated with manual hot start procedures are addressed with the use of. PLATINUM Taq DNA Polymerase. The.